Journal: Frontiers in Microbiology

Article Title: Roles of Three HSF Domain-Containing Proteins in Mediating Heat-Shock Protein Genes and Sustaining Asexual Cycle, Stress Tolerance, and Virulence in Beauveria bassiana

doi: 10.3389/fmicb.2018.01677

Figure Lengend Snippet: Contributions of Hsf1 , Sfl1 , and Skn7 to cell wall integrity of B. bassiana . (A) EC 50 for Congo red to suppress 50% colony growth of each strain after 6-day incubation at 25°C on 1/4 SDAY plates, to which small hyphal disks were attached for colony initiation. (B) Relative germination percentages of conidia after 24-h incubation at 25°C in a medium alone (control) or supplemented with Congo red (1 mg/mL). (C) Concentrations of protoplasts released from the hyphal cells after 6 h treatment with cell wall lysing enzymes in osmotic solution of 0.8 M sucrose. (D) Fluorescence intensity as an index of cell wall composition from flow cytometry of 2 × 10 4 conidia labeled with the fluorescent lectins ConA, GNL, and WGA. Asterisked bars in each bar group differ significantly from those unmarked (Tukey’s HSD, P < 0.05). Error bar: SD from three replicates.

Article Snippet: Carbohydrate epitopes on the surfaces of conidia were probed with the Alexa fluor 488-labeled lectins concanavalin A [ConA specific to α-glucose and α-N-acetylglucosamine (α-GlcNAc)], Galanthus nivalis (GNL lectin specific to mannose residues), and wheat germ agglutinin (WGA specific to β-GlcNAc and sialic acids) from Molecular Probes-Invitrogen and Vector Laboratories, respectively.

Techniques: Incubation, Fluorescence, Flow Cytometry, Labeling

Journal: bioRxiv

Article Title: The restricted nature of protein glycosylation in the mammalian brain

doi: 10.1101/2020.10.01.322537

Figure Lengend Snippet: Protein lysate from mouse cortex and cerebellum with human plasma as a positive control was treated with or without PNGase F and visualized using biotinylated lectins (Con A, GNL, PHA-E, AAL, RCA, and SNA) in addition to immunoblotting for actin and staining for total protein. Non-specific binding of lectins to PNGase F is noted by an asterisk (*) near 35 kDa. A schematic with common lectin-binding sites is shown for reference.

Article Snippet: Membranes were then incubated in 5% BSA in TBS-Tween 0.1% for 1 hour, followed by incubation with biotinylated lectins (Vector Labs: AAL B-1395, SNA B-1305, GNL B-1245, PHA-E B-1125, RCA B-1085, Con A B-1105) at a 1:1,000 dilution (1:20,000 for Con A) and 1:2,000 dilution of mouse anti-actin antibody (Abcam, ab8226) in 5% BSA in TBS-Tween 0.1%, overnight at 4°C on a rocking platform shaker.

Techniques: Positive Control, Western Blot, Staining, Binding Assay